Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Mixed infection (competition assay) by w.t. E. faecalis OG1RF and nonpiliated TX5475 in the kidneys and urinary bladder of mice (n=16). Data are expressed as log10CFU of w.t. E. faecalis OG1RF or TX5475. The log10CFU/gm from both kidneys were combined and averaged. Solid diamonds and triangles represent w.t. E. faecalis OG1RF and TX5475, respectively, from kidney homogenates and empty diamonds and triangles represent w.t. E. faecalis OG1RF and TX5475, respectively, from urinary bladder homogenates. Horizontal bars represent geometric mean titer. Mean fold difference in CFU are given. Log10 CFU were compared for statistical significance using a paired t test.

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Infection, Competitive Binding Assay

Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Tropism for the kidneys by E. faecalis strains OG1RF and its isogenic nonpiliated Δ ebpA (TX5475) mutant in a murine model of ascending urinary tract infection

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Mutagenesis, Infection

Journal:

Article Title: Importance of the Endocarditis and Biofilm-associated Pilus ( ebp ) Locus in the Pathogenesis of Enterococcus faecalis Ascending Urinary Tract Infection

doi: 10.1086/517524

Figure Lengend Snippet: Monoinfection (106, 105, 104, and 103 inocula) using w.t. E. faecalis OG1RF or nonpiliated TX5475 in the kidneys of mice (n=10). Data are expressed as log10CFU/gm of w.t. E. faecalis OG1RF or TX5475 recovered from kidney homogenates 48 hrs after transurethral challenge. The log10CFU from both kidneys were combined and averaged. Solid and empty triangles represent w.t. E. faecalis OG1RF and TX5475, respectively. Horizontal bars represent geometric mean titer. Mean difference in CFU is given as log10 ± standard deviation (SD) for the respective inocula. TX5475 CFUs (recovered from kidneys) were significantly reduced versus OG1RF with all four inocula using unpaired t test.

Article Snippet: Bacterial strains and growth conditions Bacteria used in the study include wild type OG1RF (resistant to rifampicin 100 μg/ml, fusidic acid 25 μg/ml) (ATCC 47077), a well-known plasmid-free isolate used in many laboratories [ 13 ]; OG1RF is a medium biofilm producer [ 7 ] and has been shown to be pathogenic in various animal models [ 12 , 14 - 17 ].

Techniques: Standard Deviation

Journal:

Article Title: Branched-Chain ?-Keto Acid Catabolism via the Gene Products of the bkd Operon in Enterococcus faecalis : a New, Secreted Metabolite Serving as a Temporary Redox Sink

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: These results demonstrate that pDW100 can be used as a promoter probe in E. faecalis by either histochemical screening or direct enzyme assay. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristics Reference or source Bacterial strains E. faecalis 10C1 ATCC 11700 OG1X 13 OG1RF 10 OGBKD1 BkdA − mutant of OG1RF; bkdA ::mγδ-200; Kan r 29 OGBKD2 BkdC − mutant of OG1RF; bkdC ::mγδ-200; Kan r 29 E. coli XL1 Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z ΔM15 Tn 10 (Tet r )] Stratagene Plasmids pBluescript Cloning vector; Ap r Stratagene pUC118/9 Cloning vector; Ap r 30 pDL278 Enterococcal and E. coli shuttle plasmid; Spc r 16 pNZ273 gusA reporter plasmid; Cm r 19 pNZ273e pNZ273 lacking Eco RI site This work pDW100 pDL278 containing the 2.0-kb Bam HI/ Hin dIII insert of pNZ273e containing gusA ; Spc r This work pDW101 pDW100 containing a 390-bp Bam HI/ Eco RI fragment containing the bkd promoter; Spc r This work Open in a separate window Bacterial strains and plasmids used in this study To address the possible role of the various amino and α-keto acids in the regulation of bkd expression, E. faecalis OG1RF harboring pDW101 was grown in M17 medium alone or in M17 medium containing an additional amino or α-keto acid at a concentration of 20 mM.

Techniques: Plasmid Preparation, Mutagenesis, Clone Assay